WebMay 24, 2024 · 18–24 h after plating HEK293T cells stably expressing the corresponding MiniSOG fusion construct, MiniSOG labeling was initiated by changing the medium to a fresh medium containing 500 mM biotin-phenol (Iris Biotech GMBH). It was incubated at 37 °C under 5% CO2 for 30 min. WebIris Biotech Gmbh biotin tyramide phenol Biotin Tyramide Phenol, supplied by Iris Biotech Gmbh, used in various techniques. Bioz Stars score: 86/100, based on 1 …

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For label-free experiments, Jurkat E6-1 cells (ATCC) were grown in Roswell Park Memorial Institute 1640 (RPMI) media (Gibco) supplemented with 10% fetal bovine serum (FBS) (Gibco), penicillin, and streptomycin (Gibco). For SILAC experiments human embryonic kidney 293T (HEK293) cells transfected with … See more Three biological replicates of SILAC-labeled HEK293T cells were labeled with biotin-phenol (BP) (Iris Biotech GmbH) at >80% confluency using previously described methods4. … See more Jurkat cells were lysed with 8 M urea, 50 mM Tris-HCl pH 8.0, 150 mM NaCl, 1 mM EDTA (Sigma), 2 μg/μl Aprotinin (Sigma-Aldrich), 10 μg/μl Leupeptin (Roche), and 1 mM phenylmethylsulfonyl fluoride (PMSF) (Sigma) at 4 … See more A polyclonal anti-biotin (ImmuneChem Pharmaceuticals Inc., cat no. ICP0615) was used for biotin peptide enrichment. Peptide spike-in samples were composed of varying amounts of biotinylated peptides spiked in to … See more Jurkat peptides were labeled with EZ link NHS-Biotin (Thermo Fisher Scientific). The biotin reagent was brought to RT. For labeling, 1 mg of peptide from Jurkat cell lysate was … See more WebNov 16, 2024 · Briefly, the cell medium was replaced by complete DMEM containing 500 μΜ final biotin-phenol (Iris biotech) or DMSO (control) and incubated for 30 min at 37 °C in a 5% CO 2 incubator. The APEX enzyme was induced by addition of 1 mM final H 2 O 2 diluted in 1XDPBS, in the medium, for 1 min at room temperature (RT). Following the … highnam gloucester

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WebIris Biotech Gmbh biotin tyramide phenol Biotin Tyramide Phenol, supplied by Iris Biotech Gmbh, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more WebFeb 5, 2024 · radicalized biotin-phenol moiety to both protein and RNA species. The APEX2 enzyme is reported to have a labeling radius of around 20 nm, making it suitable for the study of ... lamin-B1 construct were incubated with … WebJul 14, 2024 · Upon labeling the cells with biotin‐phenol and a short pulse of H 2 O 2, ... After 24 h of serum starvation, 500 μM of biotin‐phenol (Iris Biotech, CAS Number 41994‐02‐9) was added to the cell medium for 30 min at 37°C followed by 1 mM of H 2 O 2 for 1 min to initiate biotinylation. After 1 min, cells were washed three times with ... highnam surgery