Cytoplasmic conductivity

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August undefined, 2024

WebOur studies have revealed that K562 (human myelogenous leukemia) cells show a persistent elevation in the cytoplasmic conductivity occurring as early as 30 minutes following exposure to staurosporine. This method therefore allows a far more rapid detection method than existing biochemical marker methods. WebNov 14, 2024 · The cytoplasmic membrane integrity was analyzed by testing the relative electrical conductivity, leakage of intracellular substance, and permeation of fluorescent probe. The results indicated that the short-term adaptation improved the membrane integrity of yeast cell. The inhibition mechanism of phenolic acid might be attributed to the ...

Measurement of dielectric properties of cells at single-cell

WebFeb 9, 2024 · Low excitation voltage (10 mV) was used because higher voltages might influence the permittivity and conductivity of the cells’ cytoplasm due to the extra ion channels opened through the membrane which might influence the total complex impedance (Weaver and Chizmadzhev 1996; Jang and Wang 2007 ). WebThe activity level of Na + /K + ATPase pumps and cytoplasm ionic concentrations are directly influenced by the nutrient level, and thus, cytoplasm conductivity can be used … northern edge casino address

Specific membrane capacitance, cytoplasm conductivity …

WebJun 20, 2024 · The cytoplasm conductivity of viable cells initially remained constant and then declined from 0.40 to 0.27 S/m after 40 h, coinciding with onset of apoptotic … WebJul 9, 2024 · We conclude that cytoplasm conductivity can be used as a marker to indicate whether cells are in a recoverable state, such that the reintroduction of nutrients … WebJun 30, 2024 · Amongst these methods, electrorotation measurement is considered to be one of the most accurate method in determining the dielectric properties of cells such as … northern edge 23

Cytoplasmic Conductivity as a Marker for Bioprocess

Specific membrane capacitance, cytoplasm conductivity and

WebSep 2, 2024 · This could include the conductivity of cytoplasmic components which do not diffuse across the cell membrane, including proteins and ions with low permeability. … WebJun 15, 2024 · where KCM is the Clausius–Mossotti (CM) coefficient, σm is the conductivity of the solution, εc is the permittivity of the cell, and σc is the conductivity of the cell. The cell is mainly... how to roast a bulb of garlic in the ovenWebNov 10, 2024 · Blood samples were analyzed at 4-h intervals over 2 days by dielectrophoresis, and microscopic determination of parasitemia. We found that cytoplasmic conductivity (indicating the concentration of free ions in the cytoplasm and related to the membrane potential) exhibited circadian rhythmic behavior in all three … how to roast a brined whole chicken

"WebMar 4, 2024 · Phenotypic quantification of cells based on their plasma membrane capacitance and cytoplasmic conductivity, as determined by their dielectrophoretic frequency dispersion, is often used as a marker for their biological function. However, due to the prevalence of phenotypic heterogeneity in many biological systems of interest, there … " - Cytoplasmic conductivity

Cytoplasmic conductivity

Cytoplasm - an overview ScienceDirect Topics

WebFeb 17, 1992 · The conductivity of the cytoplasmic membrane was dependent on the conductivity of the medium. Membrane behaviour is interpreted as an opening of membrane channels when the environment becomes more physiological. The specific membrane capacitance was determined to be 1.1 microF/cm2 and the thickness of the … WebDec 7, 2024 · In other words, the steady state ionic content of the cytoplasm, which determines cytoplasmic conductivity (σ cyt), is lowered by an increased net rate of ion efflux that occurs in isolated RBCs ...

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WebJul 9, 2024 · We conclude that cytoplasm conductivity can be used as a marker to indicate whether cells are in a recoverable state, such that the reintroduction of nutrients results in cells returning to a... WebCytoplasmic Incompatibility (CI) was first recognized by the failure of certain intra- and interstrain crosses between and among various insect taxa (review: Stouthamer et al., …

WebJun 30, 2024 · Amongst these methods, electrorotation measurement is considered to be one of the most accurate method in determining the dielectric properties of cells such as their membrane capacitance as well as conductivity and permittivity of cytoplasm (Yang et al. 1999). In this method, a rotational force is applied to cells by multiple dielectrophoretic ... WebFeb 5, 2024 · Dielectric spectroscopy (DS) is a noninvasive technique for real-time measurements of the impedance spectra of biological cells. DS enables characterization of cellular dielectric properties such as membrane capacitance and cytoplasmic conductivity. We have developed a lab-on-a-chip device that uses an electro-activated microwells …

WebJun 23, 2024 · A microfluidic device enabling drug resistance analysis of leukemia cells via coupled dielectrophoretic detection and impedimetric counting Scientific Reports Article Open Access Published: 23... WebThese results indicate that as intrinsic bioelectrical markers, specific membrane capacitance and cytoplasmic conductivity can be used to classify tumor subtypes. Keywords: cytoplasmic conductivity; impedance flow cytometry; intrinsic bioelectrical marker; specific membrane capacitance; tumor subtype classification. Publication types

WebFeb 14, 2024 · In this study, single cells in suspension (adherent cells treated with trypsin) were aspirated through a microfluidic constriction channel at 25 °C, and the entry …

WebNov 10, 2016 · Pollen becomes metabolic active prior germination, thus changes in membrane integrity and cytoplasmic conductivity should be detectable when comparing germinating active with inactive pollen … northern edge casino jobsWebMay 28, 2024 · Cells were centrifuged at room temperature at 1000 rpm for 5 min and washed in a ROT solution with the conductivity of 360 mS m … northern edge cedar bowWebFeb 23, 2024 · In isotonic solutions, cytoplasmic conductivity of human erythrocyte decreases with rise in the external media ionic concentration and vice versa for … how to roast a chicken with vegetablesWebClassification of White Blood Cells Based on Cell Diameter, Specific Membrane Capacitance and Cytoplasmic Conductivity Leveraging Microfluidic Constriction Channel IEEE … northern edge casino nmWebJan 3, 2024 · These results indicate that neural net fitting can to an extent be used to estimate membrane relative permittivity and cytoplasmic conductivity based on previously unknown nine-frequency impedance … northern edge casino menuWebJun 6, 2015 · 3.3. Conductivity of the cytoplasm: regulation of the cell cytoplasm screening in response to electrical stimulation. Figure 7 shows the sensitivity of the induced EF at the membrane apex and substrate (figure 7a) and the cytoplasm (figure 7b) to the cytoplasm conductivity values. At the apex, a higher cytoplasm conductivity results … northern edge casino winnersWebFeb 1, 2024 · The measured lysate conductivity drop of 8.22% is comparable with in situ cell measurements. In comparison, the conductivity of LB medium was measured to be 1.04 ± 0.05 S/m in this work, lower than the predicted cytoplasmic conductivity of live E. coli. Thus, the dead cells are expected to loss ions to the medium, leading to a drop of … northern edge homes