Fk2 antibody

Author: dmyb

August undefined, 2024

WebUbiquitin modification of p62 was detected using an antibody recognizing mono- and poly-ubiquitin (FK2) (lower panels). The EGFP–TRIM proteins were detected using an anti-GFP antibody. * indicates mono-ubiquitylated p62 and a high-molecular-mass p62. WebImmunocytochemistry (ICC) analysis of ubiquitinylated protein conjugates using FITC labeled mono- and polyubiquitinylated conjugates mAb (UBCJ2), (Prod. No. ENZ …

Anti-Ubiquitin Mouse mAb (FK2) ST1200

WebUbiquitin Antibody - Mouse Monoclonal antibody to Ubiquitin, FK2. Species Independent. Validated for use in Western Blot, IHC, ICC/IF, IP, and ELISA. WebMay 15, 2005 · Separation of ubiquitinated Syk using an antiubiquitin antibody. Washed platelets were activated with convulxin (100 ng/mL) for 15 seconds. Cells were lysed with RIPA buffer and treated with FK2 antibody for 1 hour at 4°C, followed by Protein A/G PLUS-agarose for an additional hour. Supernatants were treated with anti-Syk using the … inbreed agency

Cyclin-Dependent Kinase 5 Regulates PSD-95 Ubiquitination in …

WebHigh Quality Antibodies Delivering Superior Results. Enzo Life Sciences offers thousands of antibodies against peptides, whole or partial proteins, and modified peptides or proteins. Backed by thousands of peer … WebRibosomal protein S27a is a component of the 40S subunit of the ribosome and belongs to the S27AE family of ribosomal proteins. It contains C4-type zinc finger domains and is … WebJan 7, 2024 · antibody product name : Polyubiquitinylated conjugates monoclonal antibody (FK1) catalog : BML-PW8805-0500 quantity : 500 µg clonality : monoclonal host : mouse conjugate : nonconjugated clone name : FK1 reactivity : spiny dogfish, human, mouse, rat, fruit fly application : western blot, ELISA, immunohistochemistry, immunocytochemistry, … inclination\u0027s dg

A versatile new tool derived from a bacterial deubiquitylase to …

Anti-Ubiquitin Antibody [FK2] Rabbit Monoclonal

WebMar 23, 2024 · The membranes were afterward incubated with mouse anti-phosphotyrosine antibody (clone 4G10; Millipore, MA, USA) diluted 1:1,000 in PBS or mouse anti-ubiquitin antibody (FK2, 1:250 dilution, monoclonal mouse antibody recognizing mono- and polyubiquitinated conjugates; ENZO Life Sciences, NY, USA) at 4°C overnight. WebFeb 21, 2014 · The third method utilized the anti-ubiquitin antibody FK2. In the initial studies, the FK2 method was the most efficient method of isolating endogenously ubiquitinated protein complexes and recognizing monoubiquitinated and … inbred youtubeWebAug 17, 2011 · The FK1 and FK2 monoclonal antibodies are used to differentiate between monoubiquitination and polyubiquitination, since FK2 detects both monoubiquitinated and polyubiquitinated proteins, while FK1 recognizes only polyubiquitinated proteins (Fujimuro et al., 1994). Therefore, to assess whether the ubiquitination of PSD-95 reflects ... inclination\u0027s dm

"WebFeb 11, 2024 · Anti-Multi Ubiquitin mAb(Monoclonal, FK2 from MBL.Clone FK2 has been reported to recognize K29-, K48-, K63-linked poly ubiquitinylated and mono … " - Fk2 antibody

Fk2 antibody

WebFeb 11, 2024 · Feb 11, 2024 in JST） Size 100 µg/100 µL Citations Data Sheet SDS Clone FK2 has been reported to recognize K29-, K48-, K63-linked poly ubiquitinylated and mono ubiquitinylated proteins but not free ubiquitin (PMID:18757370, 19237541) . The availability is based on the information in Japan at 00:05, Feb 11, 2024 in JST. WebClone FK2 Antibody Products. Clone FK2 is reported to be a monoclonal antibody specific to the human gene, UBB. Indicated applications for antibody clone FK2 include Western …

Did you know?

WebBlock: 5% Skim Milk powder in TBST. Primary Antibody: Rabbit Anti-Ubiquitin Monoclonal Antibody (SMC-550) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti … WebJun 30, 2024 · For the analysis of human cells, OtUBD pulldowns performed better than FK2 antibody immunoprecipitations in our hands, both in terms of overall efficiency and repeatability. Inconsistency in the performance of antibodies from different batches has been a widely recognized issue . Recombinant OtUBD, by contrast, appeared to be …

Web11 rows · Dilute to working strength with phosphate buffered saline pH 7.2-7.4. This Anti-Ubiquitinylated proteins Antibody, clone FK2 has been published and validated for use … Web11 rows · Anti-Ubiquitinylated proteins Antibody, clone FK2. Background Information. Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a …

WebThis Anti-Ubiquitin Mouse mAb (FK2) is validated for use in ELISA, Immunoblotting, Immunoprecipitation, Immunofluorescence for the detection of Ubiquitin. Anti-Ubiquitin Mouse mAb (FK2) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents. MSDS. 分析证书. WebThis Anti-Ubiquitin Mouse mAb (FK2) is validated for use in ELISA, Immunoblotting, Immunoprecipitation, Immunofluorescence for the detection of Ubiquitin. Anti-Ubiquitin …

WebThis Anti-Ubiquitin Mouse mAb (FK2) is validated for use in ELISA, Immunoblotting, Immunoprecipitation, Immunofluorescence for the …

WebUbiquitin Antibody (FK2) [Unconjugated] Summary. This antibody will recover mono- and polyubiquitinated proteins in I.P. applications. This antibody detects mono- and … inclination\u0027s eaWebProperties Biological Description Product Images Western Blot analysis of Purified poly-ubiquitin chains showing detection of Multiple Ubiquitin protein using Rabbit Anti-Ubiquitin Monoclonal Antibody, Clone FK2 (SMC-550). Lane 1: Molecular Weight Ladder (MW). Lane 2: K63 Poly Ubiquitin. Lane 3: K48 Poly Ubiquitin. Load: 2 µg. inclination\u0027s dxWebJan 7, 2024 · product name : Mono- and polyubiquitinylated conjugates monoclonal antibody (FK2) catalog : BML-PW8810-0100 quantity : 100 µg clonality : monoclonal host : mouse conjugate : nonconjugated clone name : FK2 reactivity : southern flounder, Saccharomycetales, human, mouse, rat, fruit fly application : inclination\u0027s dwhttp://www.utopbio.cn/archives/date/2024/04/14 inclination\u0027s egWebMar 20, 2024 · Ubiquitin staining was performed using anti-ubiquitin (FK2) antibody. The blue (Hoescht 33342) but not red bacteria are L. dumoffii. Ubiquitin was commonly associated with LpCVs (arrows), but was ... inbred yoshiWebAnti-Ubiquitinylated proteins Antibody, clone FK1 04-262 Sigma-Aldrich Anti-Ubiquitinylated proteins Antibody, clone FK1 Download Zoom Anti-Ubiquitinylated proteins Antibody, clone FK1 is an antibody against … inclination\u0027s dyWebMar 25, 2016 · We previously developed an FK2 antibody, which specifically recognizes poly-Ub moie … Polyubiquitination plays key roles in proteasome-dependent and independent cellular events, whereas monoubiquitination is involved in gene expression, DNA repair, protein-protein interaction, and protein trafficking. inclination\u0027s dr