WebTo make the MXene/HRP/Ab2 conjugation, 2 mg of MXene was dispersed into 4 mL of PBS, which was then incubated for 60 minutes with 2 mL of Ab2 solution (5 g/mL) + HRP solution (50 g/mL). GCE was cleaned and dried in the open air. The GS/TH solution (5 μL) was applied to the surface of this GCE, which was then dried. Web血清（含β淀粉样蛋白）来自北京大学肿瘤医院）；β淀粉样蛋白一抗（Aβ-Ab1）、HRP标记的二抗（HRP-Ab2）均购自Abcam公司；牛血清白蛋白（BSA）购自Sigma-Aldrich公司；氯金酸（HAuCl4·4H2O）购自南京市威圣化工贸易有限公司；柠檬酸钠购自上海创顺化工有限 …

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WebMar 5, 2024 · In the presence of PSA antigen, horseradish peroxidase secondary antibody (HRP-Ab2) was brought into the surface of the electrode, developing stable amplified signals of H 2 O 2 reduction. Under the optimal conditions, a linear curve for determination of PSA at the proposed immunosensor was 1.6×10 −4 ng.mL −1 to 38 ng.mL −1 with a limit ... WebMay 1, 2024 · Furthermore, Ab2/HRP-Au@ZnO exhibited high affinity interaction with AFP through “sandwich” immunoreactions, along with the peroxidase-like catalytic activity of … dis they draft for the navy

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WebJan 1, 2012 · The cathode peak current measured when using only HRP–Ab2 (Fig. 4 a) was slightly increased compared with the peak current measured by the HRP–avidin–biotin–Ab2 modified electrode (Fig. 4 b). This may be due to the similar hindrance of HRP molecules in both cases and the insulating effect of the avidin–biotin molecules. http://www.electrochemsci.org/papers/vol17/220712.pdf WebHRP-labeled Ab2 (HRP-Ab2) The HRP was labeled with Ab2 according to the kit instructions for activating HRP reagents. Brie y, 25 mL of Ab2 (4 mg mL−1) was transferred into a 1.5 mL tube. Then, 100 mL reagent I activated HRP (10 mg mL−1) was added. The pH of the mixture solution was adjusted to 9.5 with reagent II (about 20 mL) and cpu prioritization windows 10