Small highly active dnas that hydrolyze dna
WebbSmall, highly active DNAs that hydrolyze DNA. J. Am. Chem. Soc. 2013; 135: 9121-9129. Crossref; PubMed; Scopus (94) Google Scholar; of I-R3 in DNA cleavage. Besides I-R3, other metal-dependent deoxyribozymes that cleave a RNA bond have also been successfully engineered as molecular triggers to actively generate nanoholes in DNA origami ... Webbcorresponding / co-corresponding author * co-first author I. Eckert, R. Friedrich, C.E. Weinberg, Z. Weinberg (2024) Discovery of natural non-circular permutations in non-coding RNAs, Nucleic Acids Res, online ahead of print. F. Lenkeit, I. Eckert, M. Sinn, F. Hauth, J.S. Hartig, Z. Weinberg (2024) A variant of guanidine-IV riboswitches exhibits evidence of a …
Small highly active dnas that hydrolyze dna
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Webbhydrolyzing DNAs is further highlighted by the fact that it has been relatively straightforward to create deoxyribozymes that promote DNA cleavage by oxidative 4 or … WebbNatural DNA sequences conforming to the class I consensus sequence and structure were found that undergo hydrolysis under selection conditions (2 mM Zn2+, pH 7), which …
Webb5 juni 2013 · Natural DNA sequences conforming to the class I consensus sequence and structure were found that undergo hydrolysis under selection conditions (2 mM Zn(2+), pH 7), which demonstrates that the inherent structure of certain DNA regions might promote catalytic reactions, leading to genomic instability. Menu Log inSign up Search Papers WebbThe development of a simple DNA sensor based on DNAzyme and PCR and aimed at the detection of low concentrations of zinc (II) ions, which showed sensitivity (10 nM) and high specificity for zinc ion detection is described. AbstractThe development of a simple sensor (9NL27-Zn) based on DNAzyme and PCR and aimed at the detection of low …
Webbför 2 dagar sedan · Herein, based on the mobility change of DNA in denaturing gels, we develop a selection strategy to discover a series of 40-45 nt small DNAs that can bind … Webb6 okt. 2024 · Flow cytometry analysis. The MCF-7 cells (human breast tumor cell line), HeLa cells (cervical tumor cell line), and MCF-10A cells (human breast normal cell line) were seeded at a density of 2 × 10 5 per well onto a 6-well plate and cultured. When the cells reached 90% confluency, the F-ZD nanoparticles dispersed in the Opti-minimum …
Webb16 maj 2013 · highly-active deoxyribozymes that catalyze DNA hydrolysis indeed can be isolated. However, the precise sequence at the cleavage site and its structural …
Webb6 okt. 2024 · Herein, we propose a DNAzyme-guided DNA amplification motor through the facile built-in of a stimuli-responsive DNAzyme brake to facilitate the in vivo sensitive imaging of biomarkers by minimizing the off-site signal leakage in living systems. how do you use a suppository laxativeWebbSmall, highly active DNAs that hydrolyze DNA. Article. Full-text available. May 2013; J AM CHEM SOC; Hongzhou Gu; Kazuhiro Furukawa; Zasha Weinberg; Daniel F Berenson; Ronald R Breaker; phoning canada from germanyWebbJournal of the American Chemical Society, 135 (24), 9121–9129 10.1021/ja403585e. Gu, H., Furukawa, K., Weinberg, Z., Berenson, D. F., & Breaker, R. R. (2013). Small, Highly … how do you use a strawWebb31 maj 2024 · DNA-hydrolyzing DNAs represent an attractive type of DNA-processing catalysts distinctive from the protein-based restriction enzymes. The innate DNA property has enabled them to readily join DNA-based manipulations to promote the development of DNA biotechnology. phoning canada from irelandWebbLigation of linear single-stranded DNAs with CircLigase to form circles. CircLigase is a thermostable ATP-dependent single-stranded DNA ligase that catalyzes intramolecular ligation of single-stranded DNA templates. The ligation substrates must carry 5′-phosphate a 3′-hydroxyl groups on the donor and acceptor DNAs, respectively. phoning canada from the ukWebb(B) Hydrolytic instability of natural DNA sequences. Trace amounts of each natural DNA (5′ 32P-labeled) were incubated at 37 °C in reaction buffer without or with 2 mM Zn2+ for 6 … how do you use a strap wrenchWebbDNA phosphoester bonds are exceedingly resistant to hydrolysis in the absence of chemical or enzymatic catalysts. This property is particularly important for organisms … phoning canada from england